Generation of hydrolysates from rice bran proteins using a combined ultrasonication-Alcalase hydrolysis treatment

Hydrolysis of rice bran protein (RBP) using a physical-enzymatic combined method (ultrasound-Alcalase) was carried out. The RBP extracted an ultrasound-aided alkaline extraction and then the extract hydrolysed. Furthermore, ultrasounication used prior to Alcalase hydrolysis (pH 8.5, 10 min, 55 °C). Two variables were considered operate ultrasonication: power (50, 100 150 w) duration treatment (10, 25 40 min). results showed that degree (DH%) increased with increasing duration. highest DH (27.35 ± 0.45%) associated hydrolysates obtained by ultrasonication (150 w, min)-Alcalase treatment. optimal condition for found at 50 w-10 where bioactive peptides (BAPs) had 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH•) scavenging activity (86.55 1.17%) lipase inhibitory (57.57 0.91%). optimized studied in terms physicochemical structural properties. mass distribution materials high performance liquid chromatography (HPLC) showing lower molecular weight proteins all generated compared non-hydrolysed samples. agreement analysis sodium dodecyl sulfate polyacrylamide gel electrophoresis. Ultrasonication enhanced effect enzyme affecting structure proteins. This confirmed investigation fourier-transform infrared spectroscopy ultraviolet–visible spectrophotometry. Using ultrasound different conditions enzymatic influenced biological properties BAPs from RBP.